001、使用conda安裝bcftools， 呼叫報錯如下：
[root@PC1 ~]# conda install bcftools -c bioconda ## conda安裝 [root@PC1 ~]# bcftools ## 呼叫測試
002、解決方法1
[root@PC1 ~]# conda remove bcftools -y ## 解除安裝已安裝bcftools [root@PC1 ~]# conda install -c bioconda -c conda-forge bcftools=1.15.1 -y ## 指定版本安裝
003、測試bcftolls
[root@PC1 ~]# bcftools --version ## 可以正常呼叫 bcftools 1.15.1 Using htslib 1.16 Copyright (C) 2022 Genome Research Ltd. License GPLv3+: GNU GPL version 3 or later <http://gnu.org/licenses/gpl.html> This is free software: you are free to change and redistribute it. There is NO WARRANTY, to the extent permitted by law.
004、解決方法2
a、
[root@PC1 ~]# bcftools ## 呼叫測試 bcftools: error while loading shared libraries: libcrypto.so.1.0.0: cannot open shared object file: No such file or directory
b、
[root@PC1 ~]# find ~ -name "libcrypto.so*" ## 查詢檔案位置 /root/anaconda3/pkgs/openssl-1.1.1t-h7f8727e_0/lib/libcrypto.so /root/anaconda3/pkgs/openssl-1.1.1t-h7f8727e_0/lib/libcrypto.so.1.1 /root/anaconda3/pkgs/openssl-1.1.1u-h7f8727e_0/lib/libcrypto.so /root/anaconda3/pkgs/openssl-1.1.1u-h7f8727e_0/lib/libcrypto.so.1.1 /root/anaconda3/lib/libcrypto.so /root/anaconda3/lib/libcrypto.so.1.1 ## 建立軟連結 [root@PC1 ~]# ln -s /root/anaconda3/lib/libcrypto.so.1.1 /root/anaconda3/lib/libcrypto.so.1.0.0
[root@PC1 ~]# find ~ -name "libcrypto.so*" ## 再次查詢， 軟連結建立成功 /root/anaconda3/pkgs/openssl-1.1.1t-h7f8727e_0/lib/libcrypto.so /root/anaconda3/pkgs/openssl-1.1.1t-h7f8727e_0/lib/libcrypto.so.1.1 /root/anaconda3/pkgs/openssl-1.1.1u-h7f8727e_0/lib/libcrypto.so /root/anaconda3/pkgs/openssl-1.1.1u-h7f8727e_0/lib/libcrypto.so.1.1 /root/anaconda3/lib/libcrypto.so.1.0.0 /root/anaconda3/lib/libcrypto.so /root/anaconda3/lib/libcrypto.so.1.1
c、呼叫bcftools測試
[root@PC1 ~]# bcftools --version ## 測試版本 bcftools 1.8 Using htslib 1.8 Copyright (C) 2016 Genome Research Ltd. License Expat: The MIT/Expat license This is free software: you are free to change and redistribute it. There is NO WARRANTY, to the extent permitted by law.
轉載自:https://www.cnblogs.com/liujiaxin2018/p/17594569.html